There are many different applications that use genetic sequencing as part of their process in testing, research, and diagnosis. Having the right type of genetic sequencer in the lab that is suitable to complete the testing required is important for in-house testing and the ability to quickly access data and results.
Options to Consider
There are different options in genetic sequencer equipment. The traditional type of sequencing used in many labs is called Sanger sequencing, after the developer of the process. This is a method that uses gel electrophoresis (traditionally) or capillary electrophoresis in more recent equipment to read the bands and determine the sequence.
The other option for a genetic sequencer is the NGS or next-generation sequencing option. While both use some of the same technology, the use of NGS offers the ability to create millions of sequences on fragments that is not possible using Sanger equipment and technology.
The Best Use
There are different uses for both options, with Sanger genetic sequencer equipment seen as the “gold standard” in sequencing with its 99.99% accuracy. New options in NGS are approaching those levels very rapidly, and with higher productivity and lower costs, this is an option for any lab to consider.
There are some idea situations where Sanger sequencing is clearly the best choice. This includes when single genes are sequenced, for fragment analysis, for the identification of microbes and to confirm any results found with NGS technology.
The best use of NGS, aside from the extreme production possible in a short amount of time, can include fast cycle times to address critical issues, such as critical disease outbreaks. It is also a good choice when the emphasis is on finding as many variants as possible in a single cycle or run.
NGS is also an ideal technology to use when there are small initial sample amounts that may be difficult or impossible to test using Sanger sequencing technology.
