The Helicobacter (H) Pylori can cause chronic gastritis and peptic ulcers in human beings. It is also associated with duodenal ulcers and could be involved in the development of low-grade lymphoma of the mucosa associated with the lymphoid tissues of the stomach, as well as adenocarcinoma. You can find this antibody available for purchase so that you can run tests and research it more thoroughly. However, it shouldn’t be used for diagnostics.
More Information
The H. pylori has no known clone, and the immunogen is the purified Helicobacter pylori. The isotype is the Rabbit IgG, and it has an undetermined epitope with no available molecular weight listed.
Applications
It can be used in Immunohistochemistry applications only. To prepare the specimen, you should use a formalin-fixed or paraffin-embedded tissue sample. Deparaffinized slides are necessary and can be done using graded alcohols, xylene and any of its alternatives.
When using the concentrated formula, you should dilute the antibody using a ratio of 1:100, though this is an estimate and your testing may require different dilutions. If you do need a dilution ratio of 1:100, you can also find a pre-diluted formulation, which can help you save time and steps when testing.
When you’re ready, you can retrieve the antigen by boiling the tissue section in a 10mM Citrate buffer with a pH of 6.0 for ten minutes and then allow the concoction to cool to room temperature for 20 minutes. You should also incubate for at least 30 minutes while at room temperature.
The positive control can be any Helicobacter pylori infected tissue, and cellular localization occurs in the H. pylori, as well.
The H. pylori antibody can help you research various stomach problems in humans. Visit Spring Bioscience today for more information about purchasing.
